Skip to content

Henry Eyring Center for Cell & Genome Science

Main Navigation

 

CCGS Workshops

The Center for Cell & Genome Science is developing new techniques in cell biology, including molecular biology, fluorescence microscopy, and electron microscopy. To provide training in advanced techniques in cell biology, we are offering several intensive workshops to be scheduled during semester breaks. Fall 2021's offering is a DNA Assembly workshop to be held October 11-15, 2021.

DNA Assembly Workshop

This workshop is for any graduate student from any year who would like to brush up on their DNA chops. The class will be limited in size and will be filled on a first-come basis. 

Mornings are lectures about theory, followed by a guided tutorial for plasmid design for that day's experiment using ApE. Afternoons are in the lab assembling the plasmid. The course is oriented to graduate students from any year and will cover the following topics:
  • Day 1. Endonuclease-mediated assembly. Lab example: SapTrap assembly
  • Day 2. Polymerase-mediated assembly. Lab example: Gateway assembly
  • Day 3. Recombinase-mediated assembly. Lab example: Gateway assembly
  • Day 4. Evaluation of experiments (half day)
Biological Microscopy Workshop

This workshop comprises 1) lectures that give a conceptual foundation for modern optical microscopy and 2) hands-on lab experience applying different microscope modalities to image cells.

Lectures:  Mon-Fri. These are open to all career levels, only capped by room capacity, and do not assume prior experience. Topics will cover technical aspects of microscopy, including numerical aperture, refractive index, magnification; but also the physics of light, including fluorescence, Jablonski plots, forbidden states, phosphorescence. This information will act as the foundation for the operation of DIC, TIRF, confocal, 2-photon, and super-resolution techniques such as STED and PALM microscopy. Lectures will close with practical aspects of publishing, including experimental design, data analysis, visualization, and reporting.

Lab: Tues-Fri.The hands-on lab section is intended for graduate students and postdocs. Training will be conducted using different types of microscopes in the Cell Center. Operation of microscopes and imaging of samples will be performed using: phase and DIC; widefield and confocal fluorescence, TIRF, single-molecule super-resolution, and STED super-resolution microscopes. Enrollment is capped at 8 persons.

Last Updated: 10/12/21